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Image Search Results
Journal: PLoS ONE
Article Title: Inhibition of TGF-β Signaling Enables Human Corneal Endothelial Cell Expansion In Vitro for Use in Regenerative Medicine
doi: 10.1371/journal.pone.0058000
Figure Lengend Snippet: (A+B) Blocking the TGF-receptor signaling by SB431542 (A: 1 μM, B: 0.1, 1, and 10 μM) enabled the subcellular localization of Na + /K + -ATPase and ZO-1 at the plasma membrane and their protein expression to be maintained. Scale bar: 100 μm. (C) ELISA assay revealed that SB431542 significantly downregulated the secretion of type I collagen to the culture supernatant. **P<0.05. (D+E) Quantitative PCR showed that SB431542 significantly reduced the expression of type I collagen and fibronectin at the mRNA level. * p <0.01, ** p <0.05. Samples were prepared in duplicate. Immunoblotting, ELISA, and quantitative PCR were performed in duplicate.
Article Snippet: Collagen type I of culture medium supernatant of HCECs were measured using
Techniques: Blocking Assay, Clinical Proteomics, Membrane, Expressing, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Western Blot